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Human CA14 protein

SKU: orb391333

Description

Recombinant human CA14 protein is produced by our E. coli expression system and the target gene encoding Gly19-Met290 is expressed with a 6His tag at the N-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceE. coli
ReactivityHuman
Protein SequenceMNHKVHHHHH HMGQHWTYEG PHGQDHWPAS YPECGNNAQS PIDIQTDSVT FDPDLPALQP HGYDQPGTEP LDLHNNGHTV QLSLPSTLYL GGLPRKYVAA QLHLHWGQKG SPGGSEHQIN SEATFAELHI VHYDSDSYDS LSEAAERPQG LAVLGILIEV GETKNIAYEH ILSHLHEVRH KDQKTSVPPF NLRELLPKQL GQYFRYNGSL TTPPCYQSVL WTVFYRRSQI SMEQLEKLQG TLFSTEEEPS KLLVQNYRAL QPLNQRMVFA SFIQAGSSYT TGEMVDLQSR
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageStore it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/AppearanceLiquid in a 0.2 μM filtered solution of 20mM Tris, 150mM NaCl, 10% Glycerol, pH 8.0.
DisclaimerFor research use only

Alternative Names

Carbonic anhydrase 14; Carbonate dehydratase XIV; Carbonic anhydrase XIV; CA-XIV

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human CA14 protein (orb391333)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
DispatchUsually dispatched within 5-10 working days
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