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Catalog Number | orb391330 |
---|---|
Category | Proteins |
Description | Recombinant human CA10 protein is produced by our E. coli expression system and the target gene encoding Ala21-Asn300 is expressed with a 6His tag at the N-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 25mM Tris, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | CA10 |
Entrez | 56934 |
UniProt ID | Q9NS85 |
Protein Sequence | MNHKVHHHHH HMAQQNSPKI HEGWWAYKEV VQGSFVPVPS FWGLVNSAWN LCSVGKRQSP VNIETSHMIF DPFLTPLRIN TGGRKVSGTM YNTGRHVSLR LDKEHLVNIS GGPMTYSHRL EEIRLHFGSE DSQGSEHLLN GQAFSGEVQL IHYNHELYTN VTEAAKSPNG LVVVSIFIKV SDSSNPFLNR MLNRDTITRI TYKNDAYLLQ GLNIEELYPE TSSFITYDGS MTIPPCYETA SWIIMNKPVY ITRMQMHSLR LLSQNQPSQI FLSMSDNFRP VQPLNNRCIR TNLELQSR |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 25mM Tris, 150mM NaCl, pH 7.5. |
Alternative names | Carbonic anhydrase-related protein 10; Carbonic an Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
≥90% as determined by SDS-PAGE | |
This protein contains the human CA10(Gln22-Asn300) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
≥90% as determined by SDS-PAGE | |
This protein contains the human CA10(Ala21-Asn300) was fused with the N-terminal His Tag and expressed in E. coli. |
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