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Catalog Number | orb257363 |
---|---|
Category | Proteins |
Description | Human C-Reactive Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 224 (Accession # NP_000558.2). |
Reactivity | Human |
Tag | C-6×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 23.9 kDa |
Target | C-Reactive Protein, CRP |
UniProt ID | P02741 |
Protein Sequence | NP_000558.2 |
Source | Human C-Reactive Protein, His Tag (orb257363) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 224 (Accession # NP_000558.2). |
Expression System | HEK293 |
Biological Origin | Human |
Expression Region | Gln 19 - Pro 224 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_000558.2 |
Storage | -20℃ |
Buffer/Preservatives | Tris-NaCl buffer pH8.0 |
Alternative names | CRP,C-reactive Protein Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.9 kDa. The protein migrates as 26 kDa under reducing (R) condition (SDS-PAGE). |
Expiration Date | 6 months from date of receipt. |
Mamajan Ovezova a b , Fatma Yılmaz c , Ilgım Göktürk a , Adil Denizli Plasmonic sensing platform for C-reactive protein recognition via synthetic receptors decorated on graphene oxide and gold nanoparticles Microchemical Journal, (2024)
Meyer, Klaus et al. Targeted quantification of C-reactive protein and cystatin c and its variants by immuno-MALDI-MS Anal Chem, 86, 5807-5814 (2014)
SDS-PAGE analysis of Human C-Reactive protein
98.00% | |
26.3 kDa (predicted); 28.2 kDa (reducing conditions) |
> 95% | |
24.1 kDa (predicted). Due to glycosylation, the protein migrates to 25-27 kDa based on Tris-Bis PAGE result. |
≥90% as determined by SDS-PAGE | |
This protein contains the human CRP(Gln19-Pro224) was fused with the C-terminal His Tag and expressed in E. coli. |
> 95% by SDS-PAGE. | |
Recombinant Human C-Reactive Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Phe17-Pro224) of human C-Reactive Protein (Accession #NP_000558.2) fused with a 6��His Tag at the C-terminus. |