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Catalog Number | orb391309 |
---|---|
Category | Proteins |
Description | Recombinant human BPI protein is produced by our mammalian expression system and the target gene encoding Val32-Lys487 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | BPI |
Entrez | 671 |
UniProt ID | P17213 |
Protein Sequence | VNPGVVVRIS QKGLDYASQQ GTAALQKELK RIKIPDYSDS FKIKHLGKGH YSFYSMDIRE FQLPSSQISM VPNVGLKFSI SNANIKISGK WKAQKRFLKM SGNFDLSIEG MSISADLKLG SNPTSGKPTI TCSSCSSHIN SVHVHISKSK VGWLIQLFHK KIESALRNKM NSQVCEKVTN SVSSKLQPYF QTLPVMTKID SVAGINYGLV APPATTAETL DVQMKGEFYS ENHHNPPPFA PPVMEFPAAH DRMVYLGLSD YFFNTAGLVY QEAGVLKMTL RDDMIPKESK FRLTTKFFGT FLPEVAKKFP NMKIQIHVSA STPPHLSVQP TGLTFYPAVD VQAFAVLPNS SLASLFLIGM HTTGSMEVSA ESNRLVGELK LDRLLLELKH SNIGPFPVEL LQDIMNYIVP ILVLPRVNEK LQKGFPLPTP ARVQLYNVVL QPHQNFLLFG ADVVYKVDHH HHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | Bactericidal permeability-increasing protein; BPI; Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
52.8 kDa | |
Human Lipopolysaccharide, His Tag (orb257660) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Val 481 (Accession # P18428-1 (I465F)). |
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