Human BMP2 protein

• Catalog Number: orb80007
• Category: Proteins
• Description: BMP2 Human Recombinant produced in E.coli is homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. The BMP-2 is purified by proprietary chromatographic techniques.
• Tested applications: SDS-PAGE
• Purity: > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: BMP2
• Solubility (25°C): It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20 mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.
• Biological Origin: E.coli
• Biological Activity: 1. The ED50 as determined by the cytolysis of MC3T3-E1 cells is 50 ng/ml. 2. When implanted in the mouse thighbone muscle and grow for 14 days, Muscle tissue was harvested and examined biochemically for calcium uptake. Our unit defined as microgram calcium absorbed. The specific activity is more than 1000 Units/mg. 3. The activity of BMP-2 was tested using alkaline phosphatase, the activity in mouse muscle myoblast C2C12C ell line was found to be 1 100,000 U/mg protein. 4. The ectopic osteogenesis capacity is 1000mg fresh bone per 1mg of BMP-2.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: BMP-2 was lyophilized from concentrated (1mg/ml) sterile solution containing 20 mM NaAc pH=4.
• Alternative names: BMP-2 protein, BMP2A protein
• Note: For research use only
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of BMP-2 as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human BMP2 protein