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Catalog Number | orb80002 |
---|---|
Category | Proteins |
Description | Beta Defensin-2 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 41 amino acids and having a molecular mass of 4.3 kDa. The BD-2 is purified by proprietary chromatographic techniques. |
Tested applications | SDS-PAGE |
Purity | > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | BD 2 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized Beta Defensin-2 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P. |
Biological Origin | E.coli |
Biological Activity | Determined by the ability to chemoattract human dendritic immature cells at concentration of 10ug/ml-100ug/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The Human BD-2 was lyophilized from concentrated (1mg/ml) solution containing 20 mM PBS pH-7.4 and 130mM sodium chloride. |
Alternative names | BD-2 protein, hBD-2 protein, Defensin beta 2 prote Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human beta Defensin 2 protein
> 98% as determined by SDS-PAGE and HPLC. | |
4.3 kDa | |
E.Coli |
Greater than 90% as determined by SDS-PAGE. | |
31.3 kDa | |
E.coli |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
E. coli |
> 90% as determined by SDS-PAGE | |
22.3 kDa |