Human BDNF Protein

SKU: orb424311

Description

Recombinant of human BDNF protein

Research Area

Neuroscience

Images & Validation

• Application Notes:
  Protein content: BDNF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard

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Key Properties

• Source: Escherichia Coli
• Biological Activity: The ED50, as determined by the dose-dependent induction of C6 cells proliferation, is 1.3-2µg/ml.
• Purity: BDNF is greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
• Protein Sequence: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Storage & Handling

• Storage: Stability: Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The protein was lyophilized without any additives.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Brain-Derived Neurotrophic Factor, BDNF, MGC34632.