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| Catalog Number | orb391292 |
|---|---|
| Category | Proteins |
| Description | Recombinant human BDNF protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 119 amino acids. Bioactive BDNF is a non-covalently linked homodimer with a molecular mass of 27 kD. |
| Reactivity | Human |
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 250mM NaCl, pH 7.2. |
| Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Protein Sequence | MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR |
| UniProt ID | P23560 |
| Tested applications | ELISA, MS, SDS-PAGE, WB |
| Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
| Source | E. coli |
| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
| Alternative names | Brain-derived neurotrophic factor; BDNF; Abrineuri Read more... |
| Research Area | Epigenetics |
| Note | For research use only |
| Entrez | 627 |
| Expiration Date | 6 months from date of receipt. |
Human | |
31.25-2000pg/mL | |
12.7 pg/mL |
IHC, WB | |
Bovine, Canine, Gallus, Human, Monkey, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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