Human BDNF / Abrineurin Protein, premium grade

• Catalog Number: orb1675341
• Category: Proteins
• Description: Brain-derived neurotrophic factor (BDNF) along with nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4/5) are members of the neurotrophin family of trophic factors. The neurotrophins play essential roles in the development, survival, and function of a wide range of neurons in both the peripheral and central nervous systems. The neurotrophins interact with two cell surface receptors, the low affinity P75 receptor and the Trk family of high affinity tyrosine kinase receptors. NGF preferentially binds TrkA, BDNF and NT4/5 bind TrkB, and NT-3 binds TrkC (and TrkA to a lesser extent).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 13.5 kDa
• Target: BDNF
• Source: Human BDNF, premium grade (orb1675341) is expressed from human 293 cells (HEK293). It contains AA His 129 - Arg 247 (Accession # P23560-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human BDNF, premium grade at 1 μg/mL (100 μL/well) can bind Human TrkB, His Tag with a linear range of 2-78 ng/mL (QC tested).
• Expression Region: His 129 - Arg 247
• Endotoxins: 0.01 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 13.5 kDa. The protein migrates as 15-17 kDa and 19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human BDNF, premium grade (Cat. No. orb1675341) at 1 μg/mL (100 μL/well) can bind Human TrkB, His Tag (Cat. No. orb257719) with a linear range of 2-78 ng/mL (QC tested).