Human BDNF Protein

• Catalog Number: orb1675341
• Category: Proteins
• Description: Human BDNF Protein
• Target: BDNF
• Conjugation: Unconjugated
• Tag: Native
• Reactivity: Human
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Purity: 95%
• Protein Sequence: His 129 - Arg 247
• UniProt ID: P23560
• MW: 13.5 kDa
• Application notes: This protein carries no "tag". The protein has a calculated MW of 13.5 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression System: HEK293
• Endotoxins: 0.01 EU per μg
• Biological Origin: Human
• Biological Activity: Immobilized Human BDNF, premium grade (Cat. No. ) at 1 μg/mL (100 μL/well) can bind Human TrkB, His Tag (Cat. No. NT2-H5228) with a linear range of 2-78 ng/mL (QC tested).
• Expression Region: His 129 - Arg 247
• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Note: For research use only

Human BDNF Protein (HEK293), premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% .

iPSC derived human brain organoids, expressing neuron marker TUJ1 (green) and neuron stem cell marker NESTIN (red), were cultured for 36 days with BDNF (orb1675341) and GDNF (orb1675340). H&E staining showed brain organoids had classic neuron stem cell rossettes histology.

iPSC derived neurons forming axon net-works were cultured with differentiation protocol, including BDNF (orb1675341) and GDNF (orb1675340).

Immobilized Human BDNF Protein (HEK293), premium grade (orb1675341) at 1 μg/mL (100 μL/well) can bind Human TrkB, His Tag (orb257719) with a linear range of 2-78 ng/mL (QC tested).

Immobilized Human BDNF Protein (HEK293), premium grade (orb1675341) at 2 μg/mL (100 μL/well) can bind Human TrkB, Fc Tag (orb257720) with a linear range of 1-20 ng/mL (Routinely tested).