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Catalog Number | orb391287 |
---|---|
Category | Proteins |
Description | Recombinant human BCL2L1 protein is produced by our E.coli expression system and the target gene encoding Met1-Arg212 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 50mM KCl, 20% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | BCL2L1 |
Entrez | 598 |
UniProt ID | Q07817 |
Protein Sequence | MSQSNRELVV DFLSYKLSQK GYSWSQFSDV EENRTEAPEG TESEMETPSA INGNPSWHLA DSPAVNGATG HSSSLDAREV IPMAAVKQAL REAGDEFELR YRRAFSDLTS QLHITPGTAY QSFEQVVNEL FRDGVNWGRI VAFFSFGGAL CVESVDKEMQ VLVSRIAAWM ATYLNDHLEP WIQENGGWDT FVELYGNNAA AESRKGQERF NRLEHHHHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM HEPES, 50mM KCl, 20% Glycerol, pH 7.5. |
Alternative names | BCL2L; BCLX; Bcl-2-like protein 1; Bcl2-L-1; Apopt Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
90% | |
14.8 kDa | |
Human IL-15, His Tag (orb864189) is expressed from human 293 cells (HEK293). It contains AA Asn 49 - Ser 162 (Accession # P40933-1). |
Unconjugated | |
95% | |
24.6 kDa | |
Human Bcl-x, His Tag (orb257216) is expressed from E.coli cells. It contains AA Met 1 - Arg 212 (Accession # AAH19307). |
Unconjugated | |
92% | |
50.6 kDa | |
Human Bcl-x, GST Tag (orb257217) is expressed from E.coli cells. It contains AA Ser 2 - Arg 212 (Accession # AAH19307). |
Unconjugated | |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 49.9 kDa after removal of the signal peptide.The apparent molecular mass of BCL2L1-hFc is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Human | |
0.625 ng/mL-40 ng/mL | |
0.156 ng/mL |
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