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| Catalog Number | orb424281 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human Bcl XL protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | The protein contains 10mM Tris-HCl pH-8, 1mM EDTA and 250mM NaCl. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Application notes | Recombinant & Natural Proteins |
| Source | Escherichia Coli |
| Solubility (25°C) | Suspend BclXL in 1000µl sterile 18M-cm H2O, over night at 4°C. Dilute 10 fold into selected buffer system.Bcl-XL has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended. |
| Storage | Stability: Lyophilized Bcl-XL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-XL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Alternative names | BclXL, Bcl-X(L), Bcl-XL. |
| Note | For research use only |
Human | |
0.16-10 ng/mL | |
0.056 ng/mL |
Human | |
0.32-20ng/mL | |
0.19 ng/mL |
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 24.85 KDa. Observed: 29 KDa, reducing conditions |
0.63-40 ng/mL | |
0.15 ng/mL |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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