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Catalog Number | orb167964 |
---|---|
Category | Proteins |
Description | Recombinant of Human Bcl XL protein (His Tag) |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Solubility (25°C) | Suspend BclXL in 100?l of 0.5M Acetic acid, over night at 4°C. Dilute 10 fold into selected buffer system.Bcl-XL has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended. |
Source | Escherichia Coli |
Storage | Stability: Lyophilized Bcl-XL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Bcl-XL should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Buffer/Preservatives | The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. |
Alternative names | BclXL, Bcl-X(L), Bcl-XL. Read more... |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 6 months from date of receipt. |
89.00% | |
25.2 kDa (predicted); 30.5 kDa (reducing conditions) |
≥90% as determined by SDS-PAGE | |
This protein contains the human BCL2L1(Met1-Arg212) was fused with the C-terminal His Tag and expressed in E. coli. |