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Catalog Number | orb391285 |
---|---|
Category | Proteins |
Description | Recombinant human BCHE protein is produced by our Mammalian expression system and the target gene encoding Glu29-Leu602 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | BCHE |
Entrez | 590 |
UniProt ID | P06276 |
Protein Sequence | EDDIIIATKN GKVRGMNLTV FGGTVTAFLG IPYAQPPLGR LRFKKPQSLT KWSDIWNATK YANSCCQNID QSFPGFHGSE MWNPNTDLSE DCLYLNVWIP APKPKNATVL IWIYGGGFQT GTSSLHVYDG KFLARVERVI VVSMNYRVGA LGFLALPGNP EAPGNMGLFD QQLALQWVQK NIAAFGGNPK SVTLFGESAG AASVSLHLLS PGSHSLFTRA ILQSGSFNAP WAVTSLYEAR NRTLNLAKLT GCSRENETEI IKCLRNKDPQ EILLNEAFVV PYGTPLSVNF GPTVDGDFLT DMPDILLELG QFKKTQILVG VNKDEGTAFL VYGAPGFSKD NNSIITRKEF QEGLKIFFPG VSEFGKESIL FHYTDWVDDQ RPENYREALG DVVGDYNFIC PALEFTKKFS EWGNNAFFYY FEHRSSKLPW PEWMGVMHGY EIEFVFGLPL ERRDNYTKAE EILSRSIVKR WANFAKYGNP NETQNNSTSW PVFKSTEQKY LTLNTESTRI MTKLRAQQCR FWTSFFPKVL EMTGNIDEAE WEWKAGFHRW NNYMMDWKNQ FNDYTSKKES CVGLVDHHHH HH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
Alternative names | CHE1; Cholinesterase; Acylcholine acylhydrolase; B Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
67.1 kDa | |
Yeast |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 65.9 kDa after removal of the signal peptide. The apparent molecular mass of BCHE-His is approximately 70-130 kDa due to glycosylation. | |
Mammalian |
Unconjugated | |
95% | |
67.0 kDa | |
Human BCHE, His Tag (orb1184802) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Leu 602 (Accession # P06276-1). |
≥90% as determined by SDS-PAGE | |
This protein contains the human BCHE(Glu29-Leu602) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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