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| Catalog Number | orb424249 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human BAFF protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from 1mg/ml solution in 20 mM PBS buffer pH 7 and 0.2 M NaCl. |
| Purity | Greater than 97.0% as determined by Analysis by SDS-PAGE. |
| Protein Sequence | HHHHHHHHHH AVQGPEETVT QDCLQLIADS ETPTIQKGSY TFVPWLLSFK RGSALEEKEN KILVKETGYF FIYGQVLYTD KTYAMGHLIQ RKKVHVFGDE LSLVTLFRCI QNMPETLPNN SCYSAGIAKL EEGDELQLAI PRENAQISLD GDVTFFGALK LL |
| Application notes | Cytokines And Growth Factors |
| Source | Nicotiana benthamiana plant |
| Biological Activity | The activity is determined by dose-dependent stimulation of proliferation B cell from Human PBMC. Cell proliferation was measured by MTT method. *activity results may vary with PBMC donors. ED50 ? 50ng/ml |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized BAFF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized BAFF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BAFF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
| Alternative names | BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TN Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
Greater than 93% as determined by SDS-PAGE. | |
46.6 kDa | |
Mammalian cell |
Human | |
62.5-4000pg/mL | |
25.2 pg/mL |
> 95% as determined by SDS-PAGE and HPLC. | |
17.2 kDa | |
E.Coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.6 kDa after removal of the signal peptide.The apparent molecular mass of BAFF-R-mFc is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
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