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Catalog Number | orb257204 |
---|---|
Category | Proteins |
Description | B7-1 and B7-2, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T and Bcell responses. Although both CTLA4 and CD28 can bind to the same ligands, CTLA4 binds to B71 and B72 with a 20 100 fold higher affinity than CD28 and is involved in the downregulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas. |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 50.0 kDa |
Target | B7-1 |
Protein Sequence | NP_005182.1 |
Source | Human B7-1, Fc Tag (orb257204) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # NP_005182.1). |
Expression System | HEK293 |
Expression Region | Val 35 - Asn 242 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_005182.1 |
Storage | -20℃ |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, pH7.5 |
Alternative names | CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.0 kDa. The protein migrates as 65-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human B7-1 protein
Unconjugated | |
90% | |
25.8 kDa | |
Human B7-1, His Tag (orb257203) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1). |
Unconjugated | |
95% | |
50.9 kDa | |
Human B7-1, Mouse IgG2a Fc Tag (orb420077) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1). |
> 92% by SDS-PAGE. | |
KMP1111, Recombinant Human B7-1/CD80 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Val35-Asn242) of human B7-1/CD80 (Accession #NP_005182.1) fused with a 6×His Tag at the C-terminus. |
Biotin | |
95% | |
52.0 kDa | |
Biotinylated Human B7-1 Protein, Fc, (orb1496296) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1). |
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