Human B7-1 / CD80 Protein

• Catalog Number: orb257203
• Category: Proteins
• Description: B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 25.8 kDa
• Target: B7-1
• Protein Sequence: NP_005182.1
• Source: Human B7-1, His Tag (orb257203) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).
• Expression System: HEK293
• Expression Region: Val 35 - Asn 242
• Endotoxins: 1.0 EU per μg
• NCBI: NP_005182.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 25.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human B7-1 protein

Line graph illustrates about the sensitivity range of Human B7-1 protein using different concentrations