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Catalog Number | orb424233 |
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Category | Proteins |
Description | Recombinant of human b NGF (CHO) protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Buffer/Preservatives | The protein was lyophilized from a 0.2µm filtered solution in 20mM PB and 250mM NaCl, pH 7.0. |
Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Protein Sequence | Was analyzed by Mass spectrometry |
Application notes | Cytokines And Growth Factors |
Source | Chinese Hamster Ovary Cells |
Biological Activity | The ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be 1 x 106 units/mg. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized NGF-b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage | Stability: Lyophilized Nerve Growth Factor b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Nerve Growth Factor-beta should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC1 Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |