Human ATG3 protein

• Catalog Number: orb391267
• Category: Proteins
• Description: Recombinant human ATG3 protein is produced by our E. coli expression system and the target gene encoding Met1-His287 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
• Tested applications: ELISA, MS, SDS-PAGE, WB
• Reactivity: Human
• Form/Appearance: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Target: ATG3
• Entrez: 64422
• UniProt ID: Q9NT62
• Protein Sequence: MASMTGGQQM GRGSMQNVIN TVKGKALEVA EYLTPVLKES KFKETGVITP EEFVAAGDHL VHHCPTWQWA TGEELKVKAY LPTGKQFLVT KNVPCYKRCK QMEYSDELEA IIEEDDGDGG WVDTYHNTGI TGITEAVKEI TLENKDNIRL QDCSALCEEE EDEDEGEAAD MEEYEESGLL ETDEATLDTR KIVEACKAKT DAGGEDAILQ TRTYDLYITY DKYYQTPRLW LFGYDEQRQP LTVEHMYEDI SQDHVKKTVT IENHPHLPPP PMCSVHPCRH AEVMKKIIET VAEGGGELGV HLEHHHHHH
• Source: E. coli
• Endotoxins: Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
• Alternative names: APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG
• Note: For research use only
• Application notes: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Expiration Date: 6 months from date of receipt.