Human ATG10 protein

SKU: orb391266

Description

Recombinant human ATG10 protein is produced by our E. coli expression system and the target gene encoding Met1-Thr190 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: ATG10
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MASMTGGQQM GRGSMEEDEF IGEKTFQRYC AEFIKHSQQI GDSWEWRPSK DCSDGYMCKI HFQIKNGSVM SHLGASTHGQ TCLPMEEAFE LPLDDCEVIE TAAASEVIKY EYHVLYSCSY QVPVLYFRAS FLDGRPLTLK DIWEGVHECY KMRLLQGPWD TITQQEHPIL GQPFFVLHPC KTNEFMTPVL KNSQKINKNV NYITLEHHHH HH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APG10L; Ubiquitin-like-conjugating enzyme ATG10; Autophagy-related protein 10; APG10-like

Quick Database Links

Gene Symbol

ATG10

UniProt

Q9H0Y0

NCBI Gene ID

83734