Human ASF1A protein

SKU: orb391261

Description

Recombinant human ASF1A protein is produced by our E. coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: ASF1A
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MASMTGGQQM GRGSMAKVQV NNVVVLDNPS PFYNPFQFEI TFECIEDLSE DLEWKIIYVG SAESEEYDQV LDSVLVGPVP AGRHMFVFQA DAPNPGLIPD ADAVGVTVVL ITCTYRGQEF IRVGYYVNNE YTETELRENP PVKPDFSKLQ RNILASNPRV TRFHINWEDN TEKLEDAESS NPNLQSLLST DALPSASKGW STSENSLNVM LESHMDCMLE HHHHHH

Storage & Handling

• Storage: Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Histone chaperone ASF1A; Anti-silencing function protein 1 homolog A; hAsf1; hAsf1a; CCG1-interacting factor A; CIA; hCIA

Quick Database Links

Gene Symbol

ASF1A

UniProt

Q9Y294

NCBI Gene ID

25842