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Catalog Number | orb391261 |
---|---|
Category | Proteins |
Description | Recombinant human ASF1A protein is produced by our E. coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | ASF1A |
Entrez | 25842 |
UniProt ID | Q9Y294 |
Protein Sequence | MASMTGGQQM GRGSMAKVQV NNVVVLDNPS PFYNPFQFEI TFECIEDLSE DLEWKIIYVG SAESEEYDQV LDSVLVGPVP AGRHMFVFQA DAPNPGLIPD ADAVGVTVVL ITCTYRGQEF IRVGYYVNNE YTETELRENP PVKPDFSKLQ RNILASNPRV TRFHINWEDN TEKLEDAESS NPNLQSLLST DALPSASKGW STSENSLNVM LESHMDCMLE HHHHHH |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0. |
Alternative names | Histone chaperone ASF1A; Anti-silencing function p Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
50 kDa | |
E.coli |
≥90% as determined by SDS-PAGE | |
This protein contains the human ASF1A(Met1-Met204) was fused with the C-terminal His Tag and expressed in E. coli. |
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