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| Catalog Number | orb424145 |
|---|---|
| Category | Proteins |
| Description | Recombinant of human AREG protein |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by HPLC.(b) Analysis by SDS-PAGE. |
| Protein Sequence | SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK |
| Application notes | Cytokines And Growth Factors |
| Source | Escherichia Coli |
| Biological Activity | Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000units/mg. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized AREG in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage | Stability: Lyophilized AREG although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution AREG should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
| Alternative names | Schwannoma-derived growth factor, Colorectum cell- Read more... |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
11.3 kDa | |
E.Coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 44.9 kDa after removal of the signal peptide. The apparent molecular mass of AREG-hFc is approximately 53-70 kDa due to glycosylation. | |
Mammalian |
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