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Catalog Number | orb391223 |
---|---|
Category | Proteins |
Description | Recombinant human AMIGO2 protein is produced by our Mammalian expression system and the target gene encoding Val40-His393 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | AMIGO2 |
Entrez | 347902 |
UniProt ID | Q86SJ2 |
Protein Sequence | VCPTACICAT DIVSCTNKNL SKVPGNLFRL IKRLDLSYNR IGLLDSEWIP VSFAKLNTLI LRHNNITSIS TGSFSTTPNL KCLDLSSNKL KTVKNAVFQE LKVLEVLLLY NNHISYLDPS AFGGLSQLQK LYLSGNFLTQ FPMDLYVGRF KLAELMFLDV SYNRIPSMPM HHINLVPGKQ LRGIYLHGNP FVCDCSLYSL LVFWYRRHFS SVMDFKNDYT CRLWSDSRHS RQVLLLQDSF MNCSDSIING SFRALGFIHE AQVGERLMVH CDSKTGNANT DFIWVGPDNR LLEPDKEMEN FYVFHNGSLV IESPRFEDAG VYSCIAMNKQ RLLNETVDVT INVSNFTVSR SHAHVDHHHH HH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | ALI1; Amphoterin-induced protein 2; AMIGO-2; Alivi Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 41.7 kDa after removal of the signal peptide. The apparent molecular mass of AMIGO2-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
≥90% as determined by SDS-PAGE | |
This protein contains the human AMIGO2(Val40-His393) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
Unconjugated | |
95% | |
43.5 kDa | |
Human AMIGO2, His Tag (orb1496324) is expressed from human 293 cells (HEK293). It contains AA Val 31 - Thr 398 (Accession # Q86SJ2-1). |
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