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Catalog Number | orb391222 |
---|---|
Category | Proteins |
Description | Recombinant human AMBN protein is produced by our mammalian expression system and the target gene encoding Val27-Pro447 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | AMBN |
Entrez | 258 |
UniProt ID | Q9NP70 |
Protein Sequence | VPFFPQQSGT PGMASLSLET MRQLGSLQRL NTLSQYSRYG FGKSFNSLWM HGLLPPHSSL PWMRPREHET QQYEYSLPVH PPPLPSQPSL KPQQPGLKPF LQSAAATTNQ ATALKEALQP PIHLGHLPLQ EGELPLVQQQ VAPSDKPPKP ELPGVDFADP QGPSLPGMDF PDPQGPSLPG LDFADPQGST IFQIARLISH GPMPQNKQSP LYPGMLYVPF GANQLNAPAR LGIMSSEEVA GGREDPMAYG AMFPGFGGMR PGFEGMPHNP AMGGDFTLEF DSPVAATKGP ENEEGGAQGS PMPEANPDNL ENPAFLTELE PAPHAGLPAL PKDDIPGLPR SPSGKMKGLP SVTPAAADPL MTPELADVYR TYDADMTTSV DFQEEATMDT TMAPNSLQTS MPGNKAQEPE MMHDAWHFQE PVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | Ameloblastin Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
47.3 kDa | |
Yeast |
≥90% as determined by SDS-PAGE | |
This protein contains the human AMBN(Val27-Pro447) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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