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Human AMBN protein

SKU: orb391222

Description

Recombinant human AMBN protein is produced by our mammalian expression system and the target gene encoding Val27-Pro447 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
ReactivityHuman
Protein SequenceVPFFPQQSGT PGMASLSLET MRQLGSLQRL NTLSQYSRYG FGKSFNSLWM HGLLPPHSSL PWMRPREHET QQYEYSLPVH PPPLPSQPSL KPQQPGLKPF LQSAAATTNQ ATALKEALQP PIHLGHLPLQ EGELPLVQQQ VAPSDKPPKP ELPGVDFADP QGPSLPGMDF PDPQGPSLPG LDFADPQGST IFQIARLISH GPMPQNKQSP LYPGMLYVPF GANQLNAPAR LGIMSSEEVA GGREDPMAYG AMFPGFGGMR PGFEGMPHNP AMGGDFTLEF DSPVAATKGP ENEEGGAQGS PMPEANPDNL ENPAFLTELE PAPHAGLPAL PKDDIPGLPR SPSGKMKGLP SVTPAAADPL MTPELADVYR TYDADMTTSV DFQEEATMDT TMAPNSLQTS MPGNKAQEPE MMHDAWHFQE PVDHHHHHH
PurityGreater than 95% as determined by reducing SDS-PAGE.
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
DisclaimerFor research use only

Alternative Names

Ameloblastin

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Human AMBN protein (orb391222)

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Available Sizes

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10 μg
$ 350.00
50 μg
$ 910.00
500 μg
$ 3,540.00
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