Human AKT3 protein

SKU: orb346476

Description

Human AKT3 protein

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  Human AKT3 recombinant protein has been tested in western blot and SDS-Page and is suitable as a control for polyclonal or monoclonal anti-AKT3 in immunological assays. Akt3 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized

Key Properties

• Source: Human
• Biological Origin: Human
• Purity: Recombinant protein corresponds to amino acids 1 to 481 of mature human AKT3; Akt isoform 3. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: None. Stabilizer: 10% (v/v) Glycerol; Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10% glycerol
• Concentration: 1.03mg/ml
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

RAC, PKB, AKT, PKB gamma

SDS-PAGE of AKT3 Human Recombinant Protein. Lane 1: AKT3, unreduced. Lane 2: prestained MW markers. Lane 3: AKT3, reduced. Load: 1 µg per lane. Predicted/Observed size: 56 kDa, ~56 kDa for AKT3. Other band(s):unreduced band at 65 kDa is alternate fold for AKT3.

Western Blot of AKT3 Human Recombinant Protein Lane 1: SuperSignal MW markers. Lane2: AKT3. Load: 50 ng per lane. Primary antibody: AKT3 antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 1 hour at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637), 1 hour at room temperature. Predicted/Observed size: 56kDa, 56kDa for AKT3. Other band(s): none.

Western Blot of Rabbit AKT Antibodies. Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant: orb346473. Lane 3: AKT1, phosphatase-treated: orb346472. Lane 4: AKT1, mutant T308A/S473A: orb346474. Lane 5: AKT2, recombinant: orb346475. Lane 6: AKT2, phosphatase-treated: orb346470. Lane 7: AKT3, recombinant: orb346476. Lane 8: AKT3, phosphatase-treated: orb346471. Load: 50 ng per lane. Blot A: orb345379 Anti-Akt pT308 used at 1:2270, Blot B: orb750474 Anti-Akt used 1:1000.

Quick Database Links

UniProt

Q9Y243

RefSeq (Protein):NP_001193658.1