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Catalog Number | orb346475 |
---|---|
Category | Proteins |
Description | Human AKT2 protein |
Tested applications | SDS-PAGE, WB |
Concentration | 1.09mg/ml |
Dilution range | WB: 50ng |
Form/Appearance | Liquid |
Purity | Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2; Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. |
Conjugation | Unconjugated |
UniProt ID | P31751 |
Source | Human |
NCBI | P31751 |
Storage | Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature. |
Buffer/Preservatives | None |
Alternative names | RAC, PKB, AKT,PKB beta Read more... |
Note | For research use only |
Application notes | Human AKT2 recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized. |
Expiration Date | 6 months from date of receipt. |
Western blot analysis of Human Recombinant Protein unreduced (Lane1), prestained MW markers (Lane2), reduced. Load: 1 ug per lane (Lane3) using Human AKT2 protein
SDS-PAGE, WB | |
Unconjugated | |
Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2; Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. | |
Human |
Unconjugated | |
92% | |
59.5 kDa | |
Human Akt1, His,Strep II Tag (orb257180) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 480 (Accession # AAH00479). |
Greater than 90% as determined by SDS-PAGE. | |
71.6 kDa | |
E.coli |
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