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Catalog Number | orb244011 |
---|---|
Category | Proteins |
Description | Recombinant human RAC-beta serine/threonine-protein kinase |
Tag | N-terminal 6xHis-SUMO-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 71.6 kDa |
UniProt ID | P31751 |
Protein Sequence | NEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPLNNFSVAECQLMKTERPRPNTFVIRCLQWTTVIERTFHVDSPDEREEWMRAIQMVANSLKQRAPGEDPMDYKCGSPSDSSTTEEMEVAVSKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRHPFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEERARFYGAEIVSALEYLHSRDVVYRDIKLENLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHERLFELILMEEIRFPRTLSPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQKKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLGLLELDQRTHFPQFSYSASIRE |
Protein Length | Full Length of Mature Protein |
Source | E.coli |
Expression System | Expression Region: 2-481aa. Protein Length: Full Length of Mature Protein |
Expression Region | 2-481aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Alternative names | AKT2 Read more... |
Note | For research use only |
Application notes | Full length of His-SUMO-tag and expression region is 2-481aa |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE, WB | |
Unconjugated | |
Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2; Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. | |
Human |
Unconjugated | |
92% | |
59.5 kDa | |
Human Akt1, His,Strep II Tag (orb257180) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 480 (Accession # AAH00479). |
SDS-PAGE, WB | |
Unconjugated | |
Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2; Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. | |
Human |
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