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Catalog Number | orb424048 |
---|---|
Category | Proteins |
Description | Recombinant of human AKT1 protein |
Note | For research use only |
Application notes | Protein Kinases |
Expiration Date | 6 months from date of receipt. |
ELISA, SDS-PAGE, WB | |
Unconjugated | |
Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. | |
Human |
SDS-PAGE, WB | |
Unconjugated | |
Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. | |
Human |
SDS-PAGE, WB | |
Unconjugated | |
Recombinant protein mutant corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. There are two point mutation of key activating residues, T308A and S473A. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC. | |
Human |
Unconjugated | |
92% | |
59.5 kDa | |
Human Akt1, His,Strep II Tag (orb257180) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 480 (Accession # AAH00479). |
Greater than 90% as determined by SDS-PAGE. | |
82.7 kDa | |
E.coli |
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