Human AKT1 protein

SKU: orb346473

Description

Human AKT1 protein

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  Human AKT1 recombinant protein has been tested in SDS-Page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT1 in immunological assays. Akt1 recombinant protein is expected to be phosphorylated and in an active state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized

Key Properties

• Source: Human
• Biological Origin: Human
• Target: AKT1
• Purity: Recombinant protein corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. This protein was co-expressed with the p110 kinase domain leading to phosphorylation of key residues T308 and S473. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20°C or colder prior to use. Avoid cycles of freezing and thawing, aliquot and freeze at -20° C or colder. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) .
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: None. Stabilizer: 10% (v/v) Glycerol; Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10% glycerol
• Concentration: 1.16mg/ml
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

RAC, PKB, AKT, PKB alpha, PRKBA, activated AKT

SDS-PAGE of AKT1 Human Recombinant Protein. Lane 1: AKT1, unreduced. Lane 2: prestained MW markers. Lane 3: AKT1, reduced. Load: 1 µg per lane. Predicted/Observed size: 56 kDa, ~56 kDa for AKT1. Other band(s): none.

Western Blot of AKT1 Human Recombinant Protein. Lane 1: SuperSignal MW markers. Lane2: AKT1. Load: 50 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for 1hour at room temperature. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 1 hour at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637), 1 hour at room temperature. Predicted/Observed size: 56kDa, 56kDa for AKT1. Other band(s): none.

Western Blot of Rabbit AKT Antibodies. Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant: orb346473. Lane 3: AKT1, phosphatase-treated: orb346472. Lane 4: AKT1, mutant T308A/S473A: orb346474. Lane 5: AKT2, recombinant: orb346475. Lane 6: AKT2, phosphatase-treated: orb346470. Lane 7: AKT3, recombinant: orb346476. Lane 8: AKT3, phosphatase-treated: orb346471. Load: 50 ng per lane. Blot A: orb345379 Anti-Akt pT308 used at 1:2270, Blot B: orb750474 Anti-Akt used 1:1000.

Quick Database Links

Gene Symbol

AKT1

UniProt

P31749

RefSeq:62241011