Human AKT1 mutant (phospho-T308A) protein

• Catalog Number: orb346474
• Category: Proteins
• Description: Human AKT1 mutant (phospho-T308A) protein
• Tested applications: SDS-PAGE, WB
• Concentration: 1.1mg/ml
• Dilution range: WB: 50ng
• Form/Appearance: Liquid
• Purity: Recombinant protein mutant corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. There are two point mutation of key activating residues, T308A and S473A. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
• Conjugation: Unconjugated
• UniProt ID: P31749
• Source: Human
• NCBI: 62241011
• Storage: Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
• Buffer/Preservatives: None
• Alternative names: RAC, PKB, AKT, PKB alpha, AKT double mutant, AKT c
• Note: For research use only
• Application notes: Human AKT1 mutant recombinant protein has been tested in SDS-Page and western blot and is suitable as a control protein for immunoassays using antibodies targeting the T308 or S473 key phosphorylation sites. For western blot use at 50 ng or less. For other assays concentration is user optimized.
• Expiration Date: 6 months from date of receipt.

Western blot analysis of Human Recombinant Protein (Lane1), SuperSignal MW markers. AKT1. Load: 50 ng per lane (Lane2) using Human AKT1 mutant (phospho-T308A) protein

Western blot analysis of Human Recombinant Protein. AKT1 (S473A, T308A) unreduced (Lane1), prestained MW markers (Lane2), AKT1 (S473A, T308A), reduced. Load: 1 ug per lane (Lane3) using Human AKT1 mutant (phospho-T308A) protein