Human AKT1 mutant (phospho-T308A) protein

SKU: orb346474

Description

Human AKT1 mutant (phospho-T308A) protein

Images & Validation

• Tested Applications: SDS-PAGE, WB
• Application Notes:
  Human AKT1 mutant recombinant protein has been tested in SDS-Page and western blot and is suitable as a control protein for immunoassays using antibodies targeting the T308 or S473 key phosphorylation sites. For western blot use at 50 ng or less. For other assays concentration is user optimized

Key Properties

• Source: Human
• Biological Origin: Human
• Purity: Recombinant protein mutant corresponds to amino acids 1 to 480 of mature human AKT1; Akt isoform 1. There are two point mutation of key activating residues, T308A and S473A. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
• Form/Appearance: Liquid
• Buffer/Preservatives: None
• Concentration: 1.1mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

RAC, PKB, AKT, PKB alpha, AKT double mutant, AKT control, negative control

SDS-PAGE of AKT1 (S473A, T308A) Human Recombinant Protein. Lane 1: AKT1 (S473A, T308A) unreduced. Lane 2: prestained MW markers. Lane 3: AKT1 (S473A, T308A), reduced. Load: 1 µg per lane. Predicted/Observed size: 56 kDa, ~56 kDa for AKT1 (S473A, T308A). Other band(s): none.

Western Blot of AKT1 (S473A, T308A) Human Recombinant Protein. Lane 1: SuperSignal MW markers. Lane2: AKT1. Load: 50 ng per lane. Primary antibody: AKT1 antibody at 1:1000 for 3.5 hours at room temperature. Secondary antibody: Peroxidase mouse secondary antibody at 1:20000 for 1 hour at room temperature. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637), overnight at 4°C. Predicted/Observed size: 56kDa, 56kDa for AKT1. Other band(s): none.

Western Blot of Rabbit AKT Antibodies. Lane 1: NIR MW protein ladder. Lane 2: AKT1, recombinant: orb346473. Lane 3: AKT1, phosphatase-treated: orb346472. Lane 4: AKT1, mutant T308A/S473A: orb346474. Lane 5: AKT2, recombinant: orb346475. Lane 6: AKT2, phosphatase-treated: orb346470. Lane 7: AKT3, recombinant: orb346476. Lane 8: AKT3, phosphatase-treated: orb346471. Load: 50 ng per lane. Blot A: orb345379 Anti-Akt pT308 used at 1:2270, Blot B: orb750474 Anti-Akt used 1:1000.

Quick Database Links

UniProt

P31749

RefSeq:62241011