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Catalog Number | orb391206 |
---|---|
Category | Proteins |
Description | Recombinant human AGER protein is produced by our mmmalian expression system and the target gene encoding Ala23-Ala344 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | AGER |
Entrez | 177 |
UniProt ID | Q15109 |
Protein Sequence | AQNITARIGE PLVLKCKGAP KKPPQRLEWK LNTGRTEAWK VLSPQGGGPW DSVARVLPNG SLFLPAVGIQ DEGIFRCQAM NRNGKETKSN YRVRVYQIPG KPEIVDSASE LTAGVPNKVG TCVSEGSYPA GTLSWHLDGK PLVPNEKGVS VKEQTRRHPE TGLFTLQSEL MVTPARGGDP RPTFSCSFSP GLPRHRALRT APIQPRVWEP VPLEEVQLVV EPEGGAVAPG GTVTLTCEVP AQPSPQIHWM KDGVPLPLPP SPVLILPEIG PQDQGTYSCV ATHSSHGPQE SRAVSISIIE PGEEGPTAGS VGGSGLGTLA LAVDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
Alternative names | RAGE; Advanced glycosylation end product-specific Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
12.5 kDa | |
Human S100A14, His Tag (orb257816) is expressed from E.coli cells. It contains AA Met 1 - His 104 (Accession # Q9HCY8-1). |
Unconjugated | |
95% | |
11.5 kDa | |
Human S100B, His Tag (orb257819) is expressed from E.coli cells. It contains AA Ser 2 - Glu 92 (Accession # NP_006263). |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 34.2 kDa after removal of the signal peptide. The apparent molecular mass of RAGE-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
≥90% as determined by SDS-PAGE | |
This protein contains the human AGER(Ala23-Ala344) was fused with the C-terminal His Tag and expressed in Mammalian cells. |
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