You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1231610 |
---|---|
Category | Proteins |
Description | Human ACE2 Recombinant Protein |
Tested applications | ELISA, WB |
Tag | His, Avi tag at the C-terminus |
Dilution range | This recombinant protein can be used for SDS-PAGE. |
Form/Appearance | Lyophilized |
Purity | > 95% by SDS-PAGE. |
MW | 86.2 kDa |
Target | ACE2 |
UniProt ID | Q9BYF1 |
Protein Sequence | Gln18- Ser740 |
Source | HEK293 cells |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2-His at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike S1 with a linear range of 1.5-104.5ng/mL. |
NCBI | Q9BYF1 |
Storage | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
Buffer/Preservatives | PBS, pH 7.4 |
Alternative names | ACE-2, ACEH, ACE2 Read more... |
Note | For research use only |
Application notes | This recombinant protein can be used for SDS-PAGE. |
Expiration Date | 6 months from date of receipt. |
Immobilized human ACE2 recombinant protein at 2μg/mL (100 μL/well) can bind recombinant SARS-CoV-2 (COVID-19) spike S1 with a linear range of 1.5-104.5ng/mL.
Human ACE2 recombinant protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 95-110 kD.
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 111.2kD Observed: 115-125kD due to glycosylation | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 86.5kD Observed: 95-110kD due to glycosylation | |
HEK293 cells |
ELISA, WB | |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC | |
Predicted: 86.5kD Observed: 95-110kD due to glycosylation | |
HEK293 cells |
Greater than 85% as determined by SDS-PAGE. | |
112.5 kDa | |
Mammalian cell |
Greater than 90% as determined by SDS-PAGE. | |
112.6kDa | |
Mammalian cell |
Filter by Rating