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Catalog Number | orb423901 |
---|---|
Category | Proteins |
Description | Recombinant of human 4 1BBL protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized 4 1BBL in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE |
Source | Escherichia Coli |
Biological Activity | Fully biologically active when compared to standard. Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is 5-10ng/ml corresponding to a Specific Activity of 100,000-200,000IU/mg. |
Storage | Stability: Lyophilized 4 1BBL although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution 4 1BBL should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4. |
Alternative names | CD137L, CD137-L, 4-1BBL, 4-1BB Ligand, TNFSF9, Tum Read more... |
Note | For research use only |
Application notes | Cytokines And Growth Factors |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
25.3 kDa | |
E.coli |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |
HPLC, SDS-PAGE | |
Unconjugated | |
> 95 % by SDS-PAGE and HPLC analyses. | |
19.4 kDa |