HTR3A antibody

• Catalog Number: orb19303
• Category: Antibodies
• Description: Goat polyclonal antibody to HTR3A
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IHC
• Reactivity: Human
• Dilution range: ELISA: 1:128000, IHC-P: 3.75 μg/ml
• Conjugation: Unconjugated
• MW: 58.4 55.3 53.8
• Target: Serotonin receptor 3A / HTR3A
• Entrez: 3359
• Protein Sequence: GPQDFEKSPRDR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HTR3A antibody, anti serotonin receptor antib
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.1. Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19303 (0.3ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Small Intestine using HTR3A antibody