HTR3A Antibody

• Catalog Number: orb1246966
• Category: Antibodies
• Description: HTR3A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: C-GPQDFEKSPRDR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.2. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml. ICC/ IF:This product has been successfully used in IF/ ICC on Rat: Lu CL et al, Gastroenterology. 2009 Sep;137(3):1040-50. PMID: 19344720.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HTR3A
• UniProt ID: P46098
• NCBI: NP_001155244.1, NP_000860.2, NP_998786.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HTR3A, serotonin receptor, 5-hydroxytryptamine (se
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 55kDa band observed in Human Colon lysates (calculated MW of 55.3kDa according to NP_000860.2. Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 200kDa. This band was successfully blocked by incubation with the immunising peptide.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75ug/ml. ICC/ IF:This product has been successfully used in IF/ ICC on Rat: Lu CL et al, Gastroenterology. 2009 Sep;137(3):1040-50. PMID: 19344720.
• Expiration Date: 12 months from date of receipt.

orb1246966 (0.3 ug/ml) staining of Human Colon lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246966 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.