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Catalog Number | orb1257394 |
---|---|
Category | Antibodies |
Description | HTR2B Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 382-481 of human HTR2B (NP_000858.3). |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 54kDa |
Target | HTR2B |
UniProt ID | P41595 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | 5-hydroxytryptamine receptor 2B, 5-HT-2B, 5-HT2B, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:10 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using HTR2B antibody (orb1257394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat brain using HTR2B antibody (orb1257394) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HTR2B antibody (orb1257394) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using HTR2B antibody (orb1257394). Blue: DAPI for nuclear staining.
IF, WB | |
Bovine, Canine, Equine, Guinea pig, Porcine | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |