HSV1 Antibody / Herpes Simplex Virus Type I

• Catalog Number: orb640126
• Category: Antibodies
• Description: The antibody reacts with HSV type 1 specific antigen. It is suitable for detection of HSV in human cellular material obtained from superficial lesions or biopsies and for the early identification of HSV in infected tissue cultures. The herpes simplex virus (HSV) (also known as cold sore, night fever or fever blister) is a virus that causes a contagious disease. There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The HSV-1 strain generally appears in the orafacial organs. HSV2 usually resides in the sacral ganglion at the base of the spine. All herpes viruses are morphologically identical: they have a large double-stranded DNA genome and the virion consists of an icosahedral nucleo-capsid, which is surrounded by a lipid bilayer envelope. UL42, the processivity subunit of the HSV-1DNA polymerase binds DNA as a monomer and is essential for the replication of the virus. UL42 reduces the rate of dissociation from primer-template DNA, but it does not reduce the rate of elongation. UL42 increases the ability of UL9 to load onto DNA, thus increasing its assembly into a functional complex that is capable of unwinding duplex DNA.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Virus
• Immunogen: Baculovirus-expressed HSV DNA polymerase (POL) and POL/UL42 complex was used as the immunogen for this HSV1 antibody.
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: HSVI/2095
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This HSV1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human cervix with HSV1 antibody (clone HSVI/2095). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free HSV1 antibody (clone HSVI/2095) as confirmation of integrity and purity.