HSPD1 Antibody

• Catalog Number: orb1261747
• Category: Antibodies
• Description: HSPD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 27-240 of human Hsp60 (NP_002147.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 61kDa
• Target: HSPD1
• UniProt ID: P10809
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: 60 kDa heat shock protein, mitochondrial, 60 kDa c
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using Hsp60 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded rat kidney using Hsp60 antibody at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using Hsp60 antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug Hsp60 antibody. Western blot was performed from the immunoprecipitate using Hsp60 antibody at a dilution of 1:1000.