Hsp60/HSPD1 Antibody

• Catalog Number: orb251520
• Category: Antibodies
• Description: Hsp60/HSPD1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 61055 MW
• UniProt ID: P10809
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: 60 kDa heat shock protein, mitochondrial;60 kDa ch
• Note: For research use only
• Application notes: WB: The detection limit for Hsp60 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC(P) analysis of Mouse Intestine Tissue using Anti-Hsp60 Picoband antibody.

IHC(P) analysis of Rat Intestine Tissue using Anti-Hsp60 Picoband antibody.

IHC(P) analysis of Human Intestinal Cancer Tissue using Anti-Hsp60 Picoband antibody.

Western blot analysis using Anti-Hsp60 Picoband antibody.Lane 1:Rat Kidney Tissue;2:Mouse Kidney Tissue;3:HEPA Cell;4:NRK Cell;5:PC-12 Cell;6:SW620 Cell;7:A549 Cell;8:A431 Cell;9:HELA Cell.

IF analysis of Hsp60 using anti-Hsp60 antibody.Hsp60 was detected in immunocytochemical section of U20S cell.

IHC analysis of Hsp60 using anti-Hsp60 antibody.Hsp60 was detected in immunocytochemical section of SMMC-7721 Cell.