HSPBAP1 Rabbit Polyclonal Antibody

• Catalog Number: orb1559
• Category: Antibodies
• Description: HSPBAP1 Rabbit Polyclonal Antibody
• Target: HSPBAP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human HSPBAP1 (151-250/488aa)
• UniProt ID: Q96EW2
• MW: 54 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 27 KdA heat shock protein-associated protein 1; HB
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Rat testis), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (orb1559) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Sample: 293T Cell (human) Lysate at 40 ug, Lymph node (mouse) Lysate at 40 ug, HepG2 Cell (human) Lysate at 40 ug, Testis (mouse) Lysate at 40 ug, Primary: Anti-HSPBAP1 (orb1559) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55 kD.