HSPA5 antibody

• Catalog Number: orb334057
• Category: Antibodies
• Description: Goat polyclonal antibody to HSPA5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 0.01-0.1 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 72.3
• Target: HSPA5 / GRP78 (aa118-131)
• Entrez: 3309
• Protein Sequence: KVVEKKTKPYIQVD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: HSPA5, heat shock 70kDa protein 5 (glucose-regulat
• Note: For research use only
• Application notes: WB: Approx 75kDa band observed in lysates of cell lines HepG2, HeLa and MCF7, and in Mouse Liver and Testis lysates, and approx. 80kDa band in Rat Brain lysates (calculated MW of 72.3kDa according to Human NP_005338.1 and Rat NP_037215.1, and 72.4kDa accordin. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HepG2 (Lane 1), HeLa (Lane 2) and MCF7 (Lane 3) cell lysate using HSPA5 antibody

Western blot analysis of Mouse Liver (Lane 1) and Testes (Lane 2) and Rat Brain (Lane 3) lysate using HSPA5 antibody

Immunofluorescence analysis of HeLa cells using HSPA5 antibody