HSPA14 Antibody

• Catalog Number: orb1263648
• Category: Antibodies
• Description: HSPA14 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This Hsp 60 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human Hsp 60.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: HSPA14
• UniProt ID: Q0VDF9
• NCBI: Q0VDF9
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Heat shock 70 kDa protein 14, HSP70-like protein 1
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:25For WB starting dilution is: 1:2000
• Expiration Date: 12 months from date of receipt.

Antibody staining Hsp 60 in human pancreas sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution Lane 1: Raji whole cell lysates Lane 2: K562 whole cell lysates Lane 3: Jurkat whole cell lysates Lane 4: Hela whole cell lysates Lysates/proteins at 20 ug per lane.

Western blot analysis of Hsp 60 Antibody in mouse lung tissue lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human lung carcinoma with Hsp 60 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.