HSP90B1 antibody

• Catalog Number: orb19279
• Category: Antibodies
• Description: Goat polyclonal antibody to HSP90B1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat, Zebrafish
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5 μg/ml
• Conjugation: Unconjugated
• MW: 92.5
• Target: GPR94 / TRA1
• Entrez: 7184
• Protein Sequence: KEGVKFDESEKTKE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti TRA1 antibody, anti GRP94 antibody, anti tumo
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Liver shows staining in some hepatocyte nuclei and weak cytoplasm staining of hepatocytes. Recommended concentration, 3-5ug/ml.WB: Approx 100kDa band observed in lysates of cell line NIH/3T3 (calculated MW of 92.5kDa according to NP_003290.1). Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH3T3 cell lysate using HSP90B1 antibody

Immunohistochemical staining of Human Uterus using HSP90B1 antibody

Western blot analysis of U2OS (lane 1), HEK293 (lane 2), HeLa (lane 3) and A549 cell lysate(lane 4) using HSP90B1 antibody.

Immunofluorescence analysis of A431 cells using HSP90B1 antibody.

Immunofluorescence analysis of HeLa cells using HSP90B1 antibody.

Flow cytometric analysis of A431 cells using HSP90B1 antibody.

Immunohistochemical staining of paraffin embedded Human Liver using HSP90B1 antibody