GRP94/HSP90B1 Antibody

• Catalog Number: orb315145
• Category: Antibodies
• Description: GRP94/HSP90B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GRP94 recombinant protein (Position: R43-H221). Human GRP94 shares 99.4% and 98.9% amino acid (aa) sequence identity with mouse and rat GRP94, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 92469 MW
• UniProt ID: P14625
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Endoplasmin;94 kDa glucose-regulated protein;GRP-9
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of THP-1 cells using anti-GRP94 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of GRP94 using anti-GRP94 antibody.Lane 1:human HeLa cell; 2:human U-87MG cell; 3:human A431 cell; 4:huamn CACO-2 cell; 5:huamn THP-1 cell; 6:huamn CACO-2 cell; 7:huamn HepG2 cell; 8:huamn HL-60 cell.

WB analysis of GRP94 using anti-GRP94 antibody.Lane 1:rat PC-12 cell; 2:rat RH35 cell; 3:rat C6 cell; 4:mouse HEPA1-6 cell; 5:mouse NIH/3T3 cell; 6:mouse RAW264.7 cell; 7:mosue SP2/0 cell; 8:mouse ANA-1 cell.

IF analysis of GRP94 using anti-GRP94 antibody. GRP94 was detected in immunocytochemical section of A431 cells.

IHC analysis of GRP94 using anti-GRP94 antibody. GRP94 was detected in paraffin-embedded section of human tonsil tissue.

IHC analysis of GRP94 using anti-GRP94 antibody. GRP94 was detected in paraffin-embedded section of mouse brain tissue.

IHC analysis of GRP94 using anti-GRP94 antibody. GRP94 was detected in paraffin-embedded section of rat brain tissue.