hsp90a.1 Antibody (Center)

• Catalog Number: orb1927177
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB47443
• Tested applications: IHC-P, WB
• Reactivity: Zebrafish
• Isotype: Rabbit IgG
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 83319 Da
• Target: This hsp90a.1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 229-263 amino acids from the Central region of human (DANRE) hsp90a.1.
• UniProt ID: Q90474
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Heat shock protein HSP 90-alpha 1, hsp90a1, hsp90,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution + ZF4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysate from zebra fish heart tissue lysate, using (DANRE) hsp90a. 1 Antibody (Center). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35 ug.

Immunohistochemical analysis of paraffin-embedded Z. skeletal muscle section using hsp90a. 1 Antibody (Center). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution. Lane 1: Zebrafish muscle lysate. Lane 2: Zebrafish lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-hsp90a. 1 Antibody (Center) at 1:2000 dilution. Lane 1: Zebrafish muscle lysate. Lane 2: Zebrafish lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.