Hsp90 alpha/HSP90AA1 Antibody

• Catalog Number: orb196259
• Category: Antibodies
• Description: Hsp90 alpha/HSP90AA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Hsp90 alpha recombinant protein (Position: P2-V365). Human Hsp90 alpha shares 99% amino acid (aa) sequence identity with both mouse and rat Hsp90 alpha.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry , 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 84660 MW
• UniProt ID: P07900
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Heat shock protein HSP 90-alpha;Heat shock 86 kDa;
• Note: For research use only
• Application notes: WB: The detection limit for Hsp90 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

ICC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody.Hsp90 Alpha was detected in an immunocytochemical section of A549 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody.

ICC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody.Hsp90 Alpha was detected in an immunocytochemical section of MCF-7 cells. Biotinylated goat anti-rabbit IgG was used as secondary antibody.

Flow Cytometry analysis of A549 cells using anti-Hsp90 alpha antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody.Lane 1:human HeLa cell; 2:human Jurkat cell; 3:human A431 cell; 4:rat brain tissue; 5:rat heart tissue; 6:mouse brain tissue; 7:mouse heart tissue.

IF analysis of Hsp90 alpha using anti-Hsp90 alpha antibody. Hsp90 alpha was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody. Hsp90 Alpha was detected in a paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody. Hsp90 Alpha was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of Hsp90 Alpha using anti-Hsp90 Alpha antibody. Hsp90 Alpha was detected in a paraffin-embedded section of rat brain tissue.