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Catalog Number | orb1474047 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to HSP72 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, WB |
Reactivity | Human |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP72. The exact sequence is proprietary. |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/10 - 1/50) |
Form/Appearance | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | HSPA1A; HSPA1B |
Entrez | 3304, 3303 |
UniProt ID | P0DMV8, P0DMV9 |
Source | Mouse |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | HSPA1; HSX70; Heat shock 70 kDa protein 1A/1B; Hea Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HSP72 expression in HL60 (A) whole cell lysates. (Predicted band size: 70 kD; Observed band size: 72 kD)
Immunofluorescent analysis of HSP72 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ICC, IHC-P, IP, WB | |
Human, Monkey, Mouse, Other, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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