HSPA1A Antibody

• Catalog Number: orb1260664
• Category: Antibodies
• Description: HSPA1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70 (NP_005336.3).
• Concentration: batch dependent
• Dilution range: WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 70kDa
• Target: HSPA1A
• UniProt ID: P0DMV8
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: HSPA1A, HSP72, HSPA1, HSP70I, HSP70-1, HSP70-1A, H
• Note: For research use only
• Application notes: WB: 1:1000 - 1:3000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using HSP70 antibody (orb1260664) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human lung cancer using HSP70 antibody (orb1260664) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse testis using HSP70 antibody (orb1260664) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded rat testis using HSP70 antibody (orb1260664) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using HSP70 Polyclonal Antibody (orb1260664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.