HSP47 antibody

• Catalog Number: orb148118
• Category: Antibodies
• Description: Mouse monoclonal to DNMT1 (ATTO-488). Hsp47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of Hsp47 around fibrotic lesions. The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as a universal marker, since Hsp47 binds a single substrate. Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1C4-1A6
• Tested applications: ICC, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: Human HSP47, full length
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: ATTO-488
• MW: 47kDa
• Target: HSP47
• Entrez: 871
• UniProt ID: P50454
• NCBI: NP_001193943
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: SerpinH1 antibody, Colligin antibody, Gp46 antibod
• Note: For research use only
• Application notes: 1 µg/ml of SMC-203 was sufficient for detection of HSP47 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.