Hsp47/SERPINH1 Antibody

• Catalog Number: orb234371
• Category: Antibodies
• Description: Hsp47/SERPINH1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Hsp47 recombinant protein (Position: D247-L418). Human Hsp47 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp47.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 46441 MW
• UniProt ID: P50454
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Serpin H1;47 kDa heat shock protein;Arsenic-transa
• Note: For research use only
• Application notes: WB: The detection limit for Hsp47 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody.Lane 1:human A431 cell; 2:human SK-N-SH cell; 3:human HeLa cell; 4:rat lung tissue; 5:rat kidney tissue; 6:rat L6 cell; 7:mouse lung tissue; 8:mouse NIH/3T3 cell.

IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human breast cancer tissue.

IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human endometrial adenocarcinoma tissue.

IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue.

IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human spleen tissue.

IHC analysis of Hsp47/SERPINH1 using anti-Hsp47/SERPINH1 antibody. Hsp47/SERPINH1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue.