HSP47 antibody

• Catalog Number: orb1473922
• Category: Antibodies
• Description: Mouse monoclonal antibody to HSP47
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human HSP47. The exact sequence is proprietary.
• Dilution range: WB (1/1000 - 1/2000), IH (1/50 - 1/200)
• Form/Appearance: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: SERPINH1
• Entrez: 12406, 871
• UniProt ID: P19324, P50454
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1. Supplied in crude ascites with 0.01% sodium azide.
• Alternative names: CBP1; CBP2; HSP47; SERPINH2; Serpin H1; 47 kDa hea
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HSP47 expression in rat heart (A) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 46 kD)

Immunohistochemical analysis of HSP47 staining in human skin carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.